KA HOIKE A ME KA HOAKAKA
Hiki i ka M.pneumoniae ke hoʻoulu i ka nui o nā hōʻailona e like me ka maʻi maʻi maʻi atypical, tracheobronchitis, a me ka maʻi o ka ʻōpū hanu luna.ʻO ka tracheobronchitis ka mea maʻamau i nā keiki me ka hoʻohaʻahaʻa ʻana i ka ʻōnaehana pale, a hiki i ka 18% o nā keiki i loaʻa i ka maʻi e koi ana i ka haukapila.Ma ke kino, ʻaʻole hiki ke hoʻokaʻawale ʻia ka M. pneumoniae mai ka maʻi maʻi i hoʻokumu ʻia e nā bacteria ʻē aʻe a i ʻole nā maʻi. He mea nui kahi hōʻailona kikoʻī no ka mea ʻaʻole pono ka mālama ʻana i ka maʻi M. pneumoniae me nā lāʻau antibiotic β-lactam akā ʻo ka mālama ʻana me nā macrolides a i ʻole tetracyclines hiki ke hoʻemi i ka lōʻihi o ka maʻi.
ʻO ka pili ʻana o M. pneumoniae i ka epithelium hanu ka hana mua o ka maʻi maʻi.ʻO kēia kaʻina hana hoʻopili he hanana paʻakikī e koi ana i nā protein adhesin, e like me P1, P30, a me P116.ʻAʻole maopopo ka loaʻa ʻana o ka maʻi M. pneumoniae e pili ana i ka maʻi no ka mea paʻakikī ke ʻike i ka wā mua o ka maʻi.
KUMU
Mycoplasma Pneumoniae IgG/IgM Rapid Test Kit e pili ana i ke kumu o ka qualitative immunochromatographic assay no ka hoʻoholo ʻana i ka Mycoplasma Pneumoniae IgG/IgM antibody i loko o ka serum kanaka, plasma a i ʻole ke koko holoʻokoʻa. Aia ʻo StripA i: 1) he burgundy colored conjugate pad (me ka antigen colloid conjugate pad me ka MPMPcell conjugate antigen antigen ajugate) paʻa i loaʻa kahi pūʻali hoʻāʻo (T band) a me kahi pūʻali mana (C band).Ua uhi mua ʻia ka pūʻali T me ka antibody IgG anti-kanaka iole, a ua uhi mua ʻia ka hui C me ka anti-mouse antibody IgG antibody.Aia ka Strip B: 1) kahi papa conjugate kala burgundy i loaʻa ka antigen MP i hui pū ʻia me ke gula colloid (MP Antigen conjugates), 2) kahi ʻāpana membrane nitrocellulose i loaʻa i kahi pahu hoʻāʻo (T band) a me kahi kāʻei mana (C band).Hoʻopili mua ʻia ka pūʻali T me ka antibody IgM anti-kanaka ʻiole, a ua uhi mua ʻia ka hui C me ka anti-mouse antibody IgG antibody.
Strip A: Ke hāʻawi ʻia ka nui o nā mea hoʻāʻo i loko o ka pūnāwai hoʻāʻo o ka pahu hoʻāʻo, neʻe ka mea hoʻohālike ma o ka hana capillary ma o ka cassette.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody IgG anti-kanaka Mouse i uhi mua ʻia, e hana ana i kahi kāʻei T kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi MP IgG.ʻO ka loaʻa ʻole o ka hui T e hōʻike ana i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti-mouse IgG/mouse IgG-gold conjugate me ka nānā ʻole ʻana o ka hui T kala.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
Strip B: Ke hāʻawi ʻia ka leo kūpono o ka hōʻike hōʻike i loko o ka pūnāwai hoʻāʻo o ka pahu hoʻāʻo, neʻe ka mea hoʻohālike ma o ka hana capillary ma o ka cassette. ʻO ka antibody MP IgM inā loaʻa i ka specimen e nakinaki i nā conjugates MP Antigen.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody IgM anti-human IgM i uhi mua ʻia, e hana ana i kahi kāʻei T kala burgundy, e hōʻike ana i kahi hopena hoʻokolohua MP IgM maikaʻi.ʻO ka loaʻa ʻole o ka hui T e hōʻike ana i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti-mouse IgG/mouse IgG-gold conjugate me ka nānā ʻole ʻana o ka hui T kala.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
