ʻO ka Dengue IgG/IgM Rapid Test Kit (Colloidal Gold)

ʻO nā maʻi maʻi Dengue, he ʻohana o nā maʻi maʻi ʻehā ʻokoʻa o nā maʻi (Den 1,2,3,4), he mau maʻi maʻi RNA i hoʻopaʻa ʻia, envelop, maikaʻi.Lawe ʻia nā maʻi maʻi e nā makika o ka ʻohana Stegemyia nahu lā, ʻo ia hoʻi ʻo Aedes aegypti, a me Aedes albopictus.I kēia lā, ʻoi aku ma mua o 2.5 biliona poʻe e noho ana ma nā wahi o ʻAsia, ʻApelika, Australia, a me ʻAmelika e pilikia ana i ka maʻi dengue.Ua manaʻo ʻia he 100 miliona mau hihia o ka maʻi dengue a me 250,000 mau hihia o ka dengue hemorrhagic fever e hoʻoweliweli i ke ola i kēlā me kēia makahiki ma ke ao holoʻokoʻa1-3.

ʻO ka ʻike serological o IgM antibody ke ala maʻamau no ka ʻike ʻana i ka maʻi maʻi dengue.I kēia mau lā, ʻike ʻia nā antigens i hoʻokuʻu ʻia i ka wā o ka hoʻopiʻi hou ʻana i ka maʻi maʻi i hōʻike i ka hopena maikaʻi loa.Hiki iā ia ke hōʻoia mai ka lā mua ma hope o ka hoʻomaka ʻana o ke kuni a hiki i ka lā 9, i ka pau ʻana o ka lāʻau lapaʻau o ka maʻi, no laila e ʻae i ka mālama mua ʻana i hoʻokomo koke ʻia 4-. Hoʻokumu ʻia ka Dengue IgG/IgM Rapid Test no ka ʻike ʻana i ka hele ʻana o ka antigen dengue i ka serum , plasma a i ʻole ke koko holoʻokoʻa.Hiki ke hana ʻia ka hoʻāʻo ʻana e nā limahana i hoʻomaʻamaʻa ʻole ʻia a i ʻole ka liʻiliʻi o ka mākaukau, me ka ʻole o nā lako hana.

ʻO ka Dengue IgG/IgM Rapid Test he lateral flow chromatographic immunoassay.Aia i loko o ka pahu ho'āʻo: 1) kahi papa conjugate kala burgundy i loaʻa nā antigens envelopp recombinant dengue i hui pū ʻia me ke gula colloid (conjugates dengue) a me nā conjugates IgG-gold rabbit, 2) kahi membrane nitrocellulose i loaʻa i ʻelua mau kaula hoʻāʻo (G a me M band) a me kahi pūna mana (C band).Hoʻopili mua ʻia ka pūʻulu G me ka antibody no ka ʻike ʻana i ka maʻi anti-dengue IgG, ua uhi ʻia ka hui M me ka antibody no ka ʻike ʻana i ka maʻi anti-dengue IgM, a ua uhi mua ʻia ka hui C me ke kao anti rabbit IgG.

Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.ʻO ka maʻi anti-dengue IgG inā loaʻa i ka specimen e paʻa i nā conjugates dengue.A laila, hopu ʻia ka immunocomplex e ka reagent i uhi ʻia ma ka band G, e hana ana i kahi kāʻei G kala burgundy, e hōʻike ana i ka hopena hoʻāʻo maikaʻi o ka maʻi dengue IgG a hōʻike i kahi maʻi hou a i ʻole hou.ʻO ka maʻi anti-dengue IgM, inā loaʻa i loko o ka specimen, e hoʻopaʻa i nā conjugates dengue.A laila hopu ʻia ka immunocomplex e ka reagent i uhi mua ʻia ma ka band M, e hana ana i kahi pūʻulu M kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi ʻo dengue virus IgM a e hōʻike ana i kahi maʻi hou.

ʻO ka loaʻa ʻole o nā pūna hoʻāʻo (G a me M) e hōʻike ana i kahi hopena maikaʻi ʻole. Aia i ka hoʻāʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti rabbit IgG/rabbit IgG-gold conjugate me ka nānā ʻole i ka hoʻomohala ʻana o ka kala ma kekahi o nā kaula T.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.