KA HOIKE A ME KA HOAKAKA
ʻO Chikungunya kahi maʻi maʻi maʻi kakaʻikahi i lawe ʻia e ka nahu o ka namu Aedes aegypti i maʻi ʻia.Hōʻike ʻia ia e ka ʻeha, ke kuni, a me ka ʻeha hui nui (arthralgias) e mau ana no ʻekolu a ʻehiku lā.Loaʻa ka inoa mai ka huaʻōlelo Makonde ʻo ia hoʻi "ka mea e kulou i luna" e pili ana i ke kūlana kūlou i ulu ʻia ma muli o nā hōʻailona o ka maʻi o ka maʻi.Loaʻa ia i ka wā ua ma nā wahi wela o ka honua, ʻo ia hoʻi ma ʻApelika, South-East Asia, hema ʻo India a me Pakistan.ʻAʻole hiki ke ʻike ʻia nā hōʻailona ma ke ʻano o ka maʻi dengue.ʻOiaʻiʻo, ua hōʻike ʻia ka maʻi pālua o ka dengue a me ka chikungunya ma India.ʻAʻole e like me ka dengue, he kakaikahi nā hōʻike hemorrhagic a ʻo ka pinepine o ka maʻi he maʻi febrile palena ʻole.No laila, he mea nui ka hoʻokaʻawale ʻana i ka dengue mai ka maʻi CHIK.ʻIke ʻia ʻo CHIK ma muli o ka loiloi serological a me ka hoʻokaʻawale viral i nā ʻiole a i ʻole ka moʻomeheu kiko.ʻO ka IgM immunoassay ke ʻano hana hoʻāʻo lab.Hoʻohana ka Chikungunya IgG/IgM Rapid Test i nā antigens recombinant i loaʻa mai kona ʻano protein, ʻike ia i ka IgG/IgM anti-CHIK i ka serum maʻi a i ʻole ka plasma i loko o 20 mau minuke.Hiki ke hana ʻia ka hoʻāʻo ʻana e ka mea i hoʻomaʻamaʻa ʻole ʻia
nā limahana akamai liʻiliʻi, me ka ʻole o nā lako hana hoʻokolohua.
KUMU
ʻO ka Chikungunya IgG/IgM Rapid Test he lateral flow chromatographic immunoassay.Aia i loko o ka pahu ho'āʻo: 1) kahi papa conjugate kala burgundy i loaʻa nā antigens recombinant enveloppe Chikungunya i hui pū ʻia me ke gula colloid (conjugates dengue) a me nā conjugates IgG-gold rabbit, 2) kahi membrane nitrocellulose i loaʻa i ʻelua mau pahu hoʻāʻo (G a me M band) a me kahi pūna mana (C band).Hoʻopili mua ʻia ka pūʻulu G me ka antibody no ka ʻike ʻana i ka maʻi virus IgG anti-Chikungunya, ua uhi ʻia ka hui M me ka antibody no ka ʻike ʻana i ka maʻi maʻi IgM anti-Chikungunya, a ua uhi ʻia ka hui C me ke kao anti rabbit IgG.
Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.ʻO ka maʻi IgG anti-Chikungunya inā loaʻa i ka specimen e paʻa i nā conjugates Chikungunya.ʻAʻole hiki ke hoʻohana like ʻia nā reagents o nā helu pūʻulu like ʻole. A laila hopu ʻia ka immunocomplex e ka reagent i uhi ʻia ma ka band G, e hana ana i kahi kāʻei G kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi ʻo Chikungunya IgG a hōʻike i kahi maʻi hou a i ʻole hou.IgM anti-Chikungunya virus, inā aia i loko o ka specimen, e hoʻopaʻa i nā conjugates Chikungunya.A laila hopu ʻia ka immunocomplex e ka reagent i uhi mua ʻia ma ka band M, e hana ana i kahi hui M kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi ʻo Chikungunya virus IgM a e hōʻike ana i kahi maʻi hou.ʻO ka loaʻa ʻole o nā pūna hoʻāʻo (G a me M) e hōʻike ana i kahi hopena maikaʻi ʻole. Aia i ka hoʻāʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti rabbit IgG/rabbit IgG-gold conjugate me ka nānā ʻole i ka hoʻomohala ʻana o ka kala ma kekahi o nā kaula T.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
