wehewehe kikoʻī
ʻO ka SARS-CoV-2 Antigen Rapid Test Kit he lateral flow chromatographic immunoassay.
Aia ka pahu ho'āʻo me:
1) kahi pad conjugate kala burgundy i loaʻa i ka antigen recombinant i hui pū ʻia me ke gula colloid (monoclonal mouse anti SARS-CoV-2 antibody conjugates) a me ka rabbit IgG-gold conjugates
2) kahi ʻāpana membrane nitrocellulose i loaʻa i ka pūʻulu hoʻāʻo (nā kāʻei T) a me ka pūʻali mana (C band).
Hoʻopili mua ʻia ke kāʻei T me ka ʻiole monoclonal anti-SARS-CoV-2 NP antibody no ka ʻike ʻana i ka antigen SARS-CoV-2 NP, a ua uhi ʻia ka hui C me ke kao anti rabbit IgG.Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.ʻO ka maʻi maʻi SARS-CoV-2 inā loaʻa i ka specimen e hoʻopaʻa i ka monoclonal mouse anti- SARS-CoV-2 NP antibody conjugates.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody anti-SARS-CoV-2 NP antibody pre-coated, e hana ana i kahi kāʻei T kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi o Covid-19 NP antigen.ʻO ka loaʻa ʻole o ka pahu hoʻāʻo (T) hōʻike i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti rabbit IgG/rabbit IgG-gold conjugate me ka nānā ʻole i ka ulu ʻana o ka waihoʻoluʻu ma kekahi o nā kaula hoʻāʻo.A i ʻole, ʻaʻole kūpono ka hopena hoʻāʻo, a pono e hoʻāʻo hou ʻia ka mea hoʻohālike me kahi mea ʻē aʻe.