KA HOIKE A ME KA HOAKAKA
ʻO ka maʻi malaria kahi maʻi namu, hemolytic, febrile e hoʻopili ai ma luna o 200 miliona mau kānaka a make ma mua o 1 miliona mau kānaka i kēlā me kēia makahiki.No nā ʻano ʻehā o Plasmodium: P. falciparum, P. vivax, P. ovale, a me P. malariae.Hoʻopili kēia mau plasmodia a luku i nā erythrocytes kanaka, e hana ana i ke anu, ke kuni, ka anemia, a me ka splenomegaly.ʻO ka P. falciparum ke kumu o ka maʻi ʻoi aʻe ma mua o nā ʻano plasmodial ʻē aʻe a ʻo ia ka nui o ka make ʻana o ka maʻi malaria.ʻO P. falciparum a me P. vivax nā maʻi maʻi maʻamau, akā naʻe, nui ka hoʻololi ʻāina i ka hāʻawi ʻana i nā ʻano.
ʻO ka maʻamau, ʻike ʻia ka maʻi maʻi ma muli o ka hōʻike ʻana o nā mea ola ma Giemsa i hoʻopaʻa ʻia i nā ʻōpala mānoanoa o ke koko peripheral, a ʻokoʻa nā ʻano ʻano like ʻole o ka plasmodium e ko lākou helehelena i nā erythrocytes maʻi.Hiki i ka ʻenehana ke hōʻoia pololei a hilinaʻi hoʻi, akā ke hana wale ʻia e nā microscopists akamai me ka hoʻohana ʻana i nā protocol i wehewehe ʻia, e hōʻike ana i nā pilikia nui no nā wahi mamao a ʻilihune o ka honua.
Hoʻokumu ʻia ka Malaria Pf/Pv Ag Rapid Test no ka hoʻoponopono ʻana i kēia mau pilikia.Hoʻohana ʻo ia i nā antibodies kikoʻī iā P. falciparum Histidine Rich Protein-II (pHRP-II) a iā P. vivax Lactate Dehydrogenase (Pv-LDH) e ʻike a hoʻokaʻawale i ka maʻi me P. falciparum a me P. vivax.Hiki ke hana ʻia ka hoʻāʻo ʻana e nā limahana i hoʻomaʻamaʻa ʻole ʻia a i ʻole ka liʻiliʻi o ka mākaukau, me ka ʻole o nā lako hana
KUMU
ʻO ka Malaria Pf/Pv Ag Rapid Test he lateral flow chromatographic immunoassay.ʻO nā ʻāpana hoʻāʻo ʻāwī he: 1) kahi pad conjugate kala burgundy i loaʻa ka antibody anti-Pv-LDH ʻiole i hui pū ʻia me ke gula colloid (Pv-LDH-gold conjugates) a me ka anti-pHRP-II antibody iole i hui pū ʻia me ke gula colloid (pHRP-II-gold conjugates), 2) kahi nitrocellulose band control band (Pv) band control band (Pv) ʻelua pahu hoʻāʻo ʻo Pv.Hoʻopili mua ʻia ka pūʻali Pv me kahi ʻano anti-Pv-LDH kikoʻī ʻē aʻe no ka ʻike ʻana i ka maʻi Pv, ua uhi ʻia ka pūʻali Pf me nā antibodies polyclonal anti-pHRP-II no ka ʻike ʻana i ka maʻi Pf, a ua uhi ʻia ka hui C me ke kao anti-iole IgG.
I ka wā o ka hoʻāʻo ʻana, hāʻawi ʻia ka nui o ke koko koko i loko o ka pūnāwai hāpana (S) o ka cassette hoʻāʻo, hoʻohui ʻia kahi paʻa lysis i ka pūnāwai pale (B).Aia i loko o ka buffer kahi mea holoi e hoʻoheheʻe i nā keʻena koko ʻulaʻula a hoʻokuʻu i nā antigens like ʻole, e neʻe ana me ka hana capillary ma o ke kaula i paʻa i ka cassette.ʻO ka Pv-LDH inā loaʻa nā makana i ka specimen e hoʻopaʻa i nā conjugates Pv-LDH-gula.A laila hopu ʻia ka immunocomplex ma ka membrane e ka anti-Pv-LDH antibody i uhi mua ʻia, e hana ana i kahi pūʻulu Pv kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi Pv.ʻO kahi ʻē aʻe, pHRP-II inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates pHRP-II-gula.A laila hopu ʻia ka immunocomplex ma ka membrane e nā antibodies anti-pHRP-II i uhi mua ʻia, e hana ana i kahi pūʻulu Pf kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi Pf.ʻO ka loaʻa ʻole o nā pūʻulu hoʻāʻo e hōʻike i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi hui kala burgundy o ka immunocomplex o kao anti-iole IgG / ʻiole IgG (anti-Pv-LDH a me anti-pHRPII)-kohu gula me ka nānā ʻole i ka hoʻomohala ʻana o ka waihoʻoluʻu ma kekahi o nā kaula hoʻāʻo.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
