KA HOIKE A ME KA HOAKAKA
ʻO ka maʻi malaria kahi maʻi namu, hemolytic, febrile e hoʻopili ai ma luna o 200 miliona mau kānaka a make ma mua o 1 miliona mau kānaka i kēlā me kēia makahiki.No nā ʻano ʻehā o Plasmodium: P. falciparum, P. vivax, P. ovale, a me P. malariae.Hoʻopili kēia mau plasmodia a luku i nā erythrocytes kanaka, e hana ana i ke anu, ke kuni, ka anemia, a me ka splenomegaly.ʻO ka P. falciparum ke kumu o ka maʻi ʻoi aʻe ma mua o nā ʻano plasmodial ʻē aʻe a ʻo ia ka nui o ka make ʻana o ka maʻi malaria.ʻO P. falciparum a me P. vivax nā maʻi maʻi maʻamau, akā naʻe, nui ka hoʻololi ʻāina i ka hāʻawi ʻana i nā ʻano.
ʻO ka maʻamau, ʻike ʻia ka maʻi maʻi ma muli o ka hōʻike ʻana o nā meaola ma Giemsa i hoʻopaʻa ʻia i nā ʻōpala mānoanoa o ke koko peripheral, a ʻike ʻia nā ʻano ʻano like ʻole o ka plasmodium ma ko lākou helehelena i loko o nā erythrocytes i maʻi ʻia.Hiki i ka ʻenehana ke hōʻoia pololei a hilinaʻi hoʻi, akā ke hana wale ʻia e nā microscopists akamai me ka hoʻohana ʻana i nā protocols2 i wehewehe ʻia, e hōʻike ana i nā pilikia nui no nā wahi mamao a ʻilihune o ka honua.
Hoʻokumu ʻia ka Malaria Pf / Pan Antigen Rapid Test Kit no ka hoʻoponopono ʻana i kēia mau pilikia.Ke hoʻohana nei ka hōʻike i kahi pālua o Monoclolital a me Polyclolid antiblories i ka protein anti iparum a iʻole kekahi o nā papaʻekolu'ē aʻeʻekolu.Hiki ke hana ʻia e nā limahana i hoʻomaʻamaʻa ʻole ʻia a i ʻole ka liʻiliʻi o ka mākaukau, me ka ʻole o nā lako hana.
KUMU
ʻO ka Pf/ Pan Malaria Rapid Test kit he lateral flow chromatographic immunoassay.Aia nā ʻāpana hoʻāʻo: 1) kahi papa conjugate kala burgundy i loko o ka antibody anti- pHRP-II i hui ʻia me ke gula colloid (pHRP II-gold conjugates) a me ka anti-pLDH antibody i hui ʻia me ke gula colloid (pLDH-gold conjugates),
2) he ʻāpana membrane nitrocellulose i loaʻa i ʻelua mau pahu hoʻāʻo (Pan a me Pv band) a me kahi pūʻali mana (C band).Hoʻopili mua ʻia ka pā pan me ka antibody anti-pLDH monoclonal e ʻike ʻia ai ka maʻi me kekahi o nā ʻano ʻehā o ka plasmodia, ua uhi mua ʻia ka pā Pf me nā antibodies anti-pHRP-II polyclonal no ka ʻike ʻana i ka maʻi Pf, a ua uhi ʻia ka hui C me ke kao anti-iole IgG.
I ka wā o ka hoʻāʻo ʻana, hāʻawi ʻia ka nui o ke koko koko i loko o ka pūnāwai hāpana (S) o ka cassette hoʻāʻo, hoʻohui ʻia kahi paʻa lysis i ka pūnāwai pale (B).Aia i loko o ka buffer kahi mea holoi e hoʻoheheʻe i nā keʻena koko ʻulaʻula a hoʻokuʻu i nā antigens plasmodium like ʻole, e neʻe ana ma ka hana capillary ma o ke kaula i paʻa i ka cassette.ʻO ka pHRP-II inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates pHRP II-gula.A laila hopu ʻia ka immunocomplex ma ka membrane e nā antibodies anti-pHRPII i uhi mua ʻia, e hana ana i kahi pūʻulu Pf kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi Pf.pLDH inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates gula pLDH.A laila hopu ʻia ka immunocomplex ma ka membrane e ka antibody anti pLDH i uhi mua ʻia, e hana ana i kahi hui Pan kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi plasmodium.I ka loaʻa ʻole o Pan band, hiki ke ʻōlelo ʻia kahi hopena hōʻike maikaʻi no kekahi o nā plasmodia ʻekolu ʻē aʻe.
ʻO ka loaʻa ʻole o nā pūna hoʻāʻo (Pan a me Pf) hōʻike i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti-mouse IgG / mouse IgG (pHRP-II a me pLDH-gold conjugates) me ka nānā ʻole i ka hoʻomohala ʻana o ka waihoʻoluʻu ma kekahi o nā kaula hoʻāʻo.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
