KA HOIKE A ME KA HOAKAKA
ʻO ka maʻi malaria kahi maʻi namu, hemolytic, febrile e hoʻopili ai ma luna o 200 miliona mau kānaka a make ma mua o 1 miliona mau kānaka i kēlā me kēia makahiki.No nā ʻano ʻehā o Plasmodium: P. falciparum, P. vivax, P. ovale, a me P. malariae.Hoʻopili kēia mau plasmodia a luku i nā erythrocytes kanaka, e hana ana i ke anu, ke kuni, ka anemia, a me ka splenomegaly.ʻO ka P. falciparum ke kumu o ka maʻi koʻikoʻi ma mua o nā ʻano plasmodial ʻē aʻe a ʻo ia ka nui o ka make ʻana o ka maʻi malaria, a ʻo ia kekahi o nā maʻi maʻi maʻi maʻi maʻamau ʻelua.
ʻO ka maʻamau, ʻike ʻia ka maʻi maʻi ma muli o ka hōʻike ʻana o nā mea ola ma Giemsa i hoʻopaʻa ʻia i nā ʻōpala mānoanoa o ke koko peripheral, a ʻokoʻa nā ʻano ʻano like ʻole o ka plasmodium e ko lākou helehelena i nā erythrocytes maʻi.Hiki i ka ʻenehana ke hōʻoia pololei a hilinaʻi hoʻi, akā ke hana wale ʻia e nā microscopists akamai me ka hoʻohana ʻana i nā protocol i wehewehe ʻia, e hōʻike ana i nā pilikia nui no nā wahi mamao a ʻilihune o ka honua.
Hoʻokumu ʻia ka Pf Ag Rapid Test no ka hoʻoponopono ʻana i kēia mau pilikia.ʻIke ia i ka Pf kikoʻī antigen pHRP-II i loko o ke koko kanaka.Hiki ke hana ʻia e nā limahana i hoʻomaʻamaʻa ʻole ʻia a i ʻole ka liʻiliʻi o ka mākaukau, me ka ʻole o nā lako hana.
KUMU
ʻO ka Pf Ag Rapid Test he lateral flow chromatographic immunoassay.Aia nā ʻāpana hoʻāʻo: 1) kahi pad conjugate kala burgundy i loaʻa ka antibody anti-pHRP-II monoclonal i hui pū ʻia me ke gula colloid (pHRP II-gold conjugates), 2) kahi membrane nitrocellulose i loaʻa i kahi pahu hoʻāʻo (Pf) a me kahi kāʻei mana (C band).Hoʻopili mua ʻia ka pūʻulu Pf me nā antibodies anti-pHRP-II polyclonal, a ua uhi mua ʻia ka hui C me ka IgG anti-iole kao.
I ka wā o ka hoʻāʻo ʻana, hāʻawi ʻia ka nui o ke koko koko i loko o ka pūnāwai hāpana (S) o ka cassette hoʻāʻo, hoʻohui ʻia kahi paʻa lysis i ka pūnāwai pale (B).Aia i loko o ka pahu kahi mea holoi e hoʻoheheʻe i nā keʻena koko ʻulaʻula a hoʻokuʻu i ka antigen pHRPII, e neʻe ana ma o ka hana capillary ma o ke kaula i paʻa i ka cassette.ʻO ka pHRP-II inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates pHRP II-gula.A laila hopu ʻia ka immunocomplex ma ka membrane e nā antibodies polyclonal antiPHRP II i uhi mua ʻia, e hana ana i kahi pūʻulu Pf kala burgundy, e hōʻike ana i kahi hopena hoʻokolohua maikaʻi.
ʻO ka loaʻa ʻole o ka hui Pf hōʻike i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti-mouse IgG / mouse IgG (pHRP II-gold conjugates) me ka nānā ʻole i ka hoʻomohala ʻana o ka waihoʻoluʻu ma kekahi o ka hui Pf.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
