wehewehe kikoʻī
Inā loaʻa kahi nui o ka antibody HIV-1 a i ʻole HIV-2 antibody i loko o ka serum, ʻo ka antibody HIV i ka serum a me ka recombinant gp41 antigen a me gp36 antigen i loko o ka lepili gula e immunoconjugated e hana i kahi paʻakikī i ka wā chromatography i ke kūlana gula.Ke hōʻea ka chromatography i ka laina hoʻāʻo (laina T1 a i ʻole laina T2), e immunoconjugated ka paʻakikī me ka recombinant gp41 antigen i hoʻokomo ʻia i ka laina T1 a i ʻole ka recombinant gp36 antigen i hoʻokomo ʻia i ka laina T2, no laila e kala ʻia ke gula colloidal bridging ma ka laina T1 a i ʻole laina T2.Ke hoʻomau ʻia ka chromatographed o nā lepili gula i koe i ka laina hoʻomalu (laina C), e kala ʻia ka lepili gula e ka hopena immune me ka multiantibody i hoʻokomo ʻia ma ʻaneʻi, ʻo ia hoʻi, ʻo ka laina T a me ka laina C e waihoʻoluʻu e like me nā kaula ʻulaʻula, e hōʻike ana aia ka antibody HIV i loko o ke koko;Inā ʻaʻole i loaʻa i ka serum ka antibody HIV a i ʻole ka liʻiliʻi ma mua o kahi nui, ʻaʻole e pane ka recombinant gp41 antigen a i ʻole gp36 antigen ma T1 a i ʻole T2, a ʻaʻole e hōʻike ka laina T i ke kala, ʻoiai ʻo ka polyclonal antibody ma C laina e hōʻike i ka waihoʻoluʻu ma hope o ka hopena immune me ka lepili gula, e hōʻike ana ʻaʻohe antibody HIV i loko o ke koko.