KA HOIKE A ME KA HOAKAKA
ʻO ka lymphatic filariasis i kapa ʻia ʻo Elephantiasis, ka mea nui i hoʻokumu ʻia e W. bancrofti a me B. malayi, pili i kahi 120 miliona mau kānaka ma luna o 80 mau ʻāina.Hoʻouna ʻia ka maʻi i ke kanaka ma o ka nahu ʻana o nā makika i hoʻopili ʻia i loko o ka microflariae i omo mai ke kanaka i loaʻa i ka maʻi i ulu aʻe i loko o ke kolu o ka pae.ʻO ka maʻamau, pono ka ʻike pinepine ʻana a me ka lōʻihi i nā ʻōpala i hoʻopili ʻia no ka hoʻokumu ʻana i ka maʻi kanaka.
ʻO ka hōʻailona parasitologic definitive ka hōʻike ʻana o ka microflariae i nā laʻana koko.Eia nō naʻe, ua kaupalena ʻia kēia hoʻokolohua maʻamau gula e ke koi no ka hōʻiliʻili koko i ka pō a me ka nele o ka naʻau.Loaʻa i ke kālepa ka ʻike ʻana i nā antigens e holo ana.Ua kaupalena 'ia kona pono no W. bancrofti.Eia kekahi, ulu ka microfilaremia a me ka antigenemia mai nā mahina a i nā makahiki ma hope o ka hoʻolaha ʻana.
Hāʻawi ka ʻike antibody i kahi ala mua e ʻike ai i ka maʻi filarial parasite.ʻO ka loaʻa ʻana o ka IgM i nā antigens parasite e hōʻike ana i ka maʻi o kēia manawa, akā, pili ka IgG i ka pae hope o ka maʻi a i ʻole ka maʻi i hala.Eia kekahi, ʻo ka ʻike ʻana i nā antigens i mālama ʻia e hiki ke hoʻohana ʻia ka ho'āʻo 'pan-filaria'.Hoʻopau ka hoʻohana ʻana i nā protein recombinant i ka cross-rection me nā poʻe me nā maʻi parasitic ʻē aʻe.
Hoʻohana ka Filariasis IgG/IgM Combo Rapid Test i ka recombinant i mālama ʻia
nā antigens e ʻike like i ka IgG a me IgM i ka W. bancrofti a me B. malayi parasites me ka ʻole o ke kaohi ʻana i ka hōʻiliʻili specimen.
KUMU
ʻO ka Filariasis IgG/IgM Rapid Test Kit he lateral flow chromatographic immunoassay.Aia i loko o ka pahu ho'āʻo: 1) kahi papa conjugate kala burgundy i loaʻa nā antigens maʻamau recombinant W. bancrofti a me B. malayi i hui pū ʻia me ke gula colloid (Filariasis conjugates) a me nā conjugates IgG-gula rabbit, 2) kahi ʻāpana nitrocellulose membrane i loaʻa i ʻelua mau kaula hoʻāʻo (M a me G a me ke kaula hoʻāʻo).Hoʻopili muaʻia ka M band me ka monoclonal anti-human IgM no kaʻikeʻana i ka IgM anti-W. bancrofti a me B. malayi, ua uhi muaʻia ka G band me nā reagents no kaʻikeʻana i ka IgG anti-W.bancrofti a me B. malayi, a ua uhi mua ʻia ka hui C me ke kao anti rabbit IgG.
Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette, neʻe ka mea hōʻike ma ka hana capillary ma waena o ka cassette.ʻO W. bancrofti a i ʻole B. malayi IgM antibodies inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates Filariasis.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody IgM anti-human precoated, e hana ana i kahi pahu M kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi W. bancrofti a i ʻole B. malayi IgM.
ʻO W. bancrofti a i ʻole B. malayi IgG antibodies inā loaʻa i ka specimen e paʻa i nā conjugates Filariasis.A laila, hopu ʻia ka immunocomplex e nā reagents i uhi mua ʻia ma ka membrane, e hana ana i kahi hui G kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi W. bancrofti a i ʻole B. malayi IgG.
ʻO ka loaʻa ʻole o nā pūna hoʻāʻo (M a me G) hōʻike i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti rabbit IgG/rabbit IgG-gold conjugate me ka nānā ʻole i ka ulu ʻana o ka waihoʻoluʻu ma kekahi o nā kaula hoʻāʻo.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
