wehewehe kikoʻī
Hoʻohana ʻo Canine parvovirus antigen rapid test kit i ke kumumanaʻo o ke ʻano hana sandwich pālua antibody e ʻike pono ai i ka canine parvovirus antigen i nā feces ʻīlio.Ua hoʻohana ʻia ka ʻīlio maʻamau parvovirus antibody 1 ma ke ʻano he hōʻailona hōʻailona, a ʻo ka ʻāpana ʻike (T) a me ka ʻāpana hoʻomalu (C) ma ka membrane nitrocellulose ua uhi ʻia me ka canine parvovirus antibody 2 a me ka hipa anti-moa.I ka manawa o ka ʻike ʻana, he chromatographic ka hāpana ma lalo o nā hopena capillary.Inā loaʻa i ka laʻana i hoʻāʻo ʻia he antigen canine parvovirus, hoʻokumu ʻia ka antibody 1 maʻamau gula i kahi antigen-antibody complex me ka canine parvovirus, a hui pū me ka canine parvovirus antibody 2 i hoʻopaʻa ʻia i loko o ka wahi ʻike i ka wā chromatography e hana i kahi "antibody 1-antigen-antibody 2" sandwich, e loaʻa ai kahi hui poni-ʻulaʻula ma ka wahi ʻike (T);ʻO ka mea ʻē aʻe, ʻaʻole ʻike ʻia nā kaula poni-ʻulaʻula ma ka ʻāpana ʻike (T);Me ka nānā ʻole ʻana o ka canine parvovirus antigen i loko o ka laʻana, e hoʻomau ʻia ka paʻakikī IgY o ka moa maʻamau gula i luna i ka ʻāpana hoʻomalu (C), a e ʻike ʻia kahi pūʻulu ʻulaʻula ʻulaʻula.ʻO ke kāʻei poni-ʻulaʻula i hōʻike ʻia ma ka ʻāpana hoʻomalu (C) ka maʻamau no ka hoʻoholo ʻana inā he maʻamau ke kaʻina hana chromatography, a lawelawe pū kekahi ma ke ʻano o ka mana kūloko no nā reagents.