KA HOIKE A ME KA HOAKAKA
ʻO Chlamydia pneumoniae (C. pneumoniae) kekahi ʻano bacteria maʻamau a he kumu nui o ka maʻi maʻi maʻi a puni ka honua.Ma kahi o 50% o nā mākua i loaʻa nā hōʻike o ka maʻi i hala ma mua o 20 makahiki, a he mea maʻamau ka maʻi hou ma hope o ke ola.He nui nā haʻawina i manaʻo i ka pilina pololei ma waena o ka maʻi C. pneumoniae a me nā maʻi ʻeha ʻē aʻe e like me atherosclerosis, exacerbations acute o COPD, a me ka hānō.
He mea paʻakikī ka ʻike ʻana i ka maʻi C. pneumoniae ma muli o ke ʻano wikiwiki o ka pathogen, ka nui o ka seroprevalence, a me ka hiki ke loaʻa ke kaʻa asymptomatic transient.Hoʻokumu ʻia nā ʻano hana hoʻokolohua diagnostic ʻo ka hoʻokaʻawale ʻana o ke kino i ka moʻomeheu cell, serological assays a me PCR.ʻO nā antibody immunoassays nā hoʻokolohua serology maʻamau i hoʻohana ʻia a ʻike ʻia ka maʻi chlamydial mua e ka pane IgM nui i loko o 2 a 4 mau pule a me kahi pane IgG a me IgA lohi i loko o 6 a 8 mau pule.Eia nō naʻe, i ka hoʻomaʻamaʻa hou ʻana, piʻi koke nā pae IgG a me IgA, pinepine i loko o 1-2 pule akā ʻaʻole hiki ke ʻike ʻia nā pae IgM.No kēia kumu, ua hōʻike ʻia nā antibodies IgA he hōʻailona immunological hilinaʻi o nā maʻi mua, maʻi mau a me nā maʻi hou i ka wā i hui pū ʻia me ka ʻike ʻana o IgM.
KUMU
Chlamydia pneumoniae IgG/IgM Rapid Test kit ua hoʻokumu ʻia ma ke kumu o ka qualitative immunochromatographic assay no ka hoʻoholo ʻana i ka Chlamydia pneumoniae IgG/IgM antibody i loko o ke kanaka serum, plasma a i ʻole ke koko holoʻokoʻa. ʻO ka StripA he: 1) kahi papa conjugate kala burgundy me C. pneumoniae colloidgen conjugates conjugates (conjugated colloidgen conjugates) gula conjugated C. pneumoniae. e nalowale i ke kaula membrane i loaʻa kahi pūʻā hoʻāʻo (T band) a me kahi pūʻali mana (C band).Ua uhi mua ʻia ka hui T me ka antibody IgG anti-human, a ua uhi mua ʻia ka hui C me ka anti-mouse antibody IgG antibody.Aia ka Strip B: 1) he papa conjugate kala burgundy i loko o ka C. pneumoniae antigen i hui pū ʻia me ke gula colloid (C. pneumoniae Antigen conjugates), 2) a
nitrocellulose membrane strip i loaʻa kahi pūʻā hoʻāʻo (T band) a me kahi pūʻali mana (C band).Ua uhi mua ʻia ka hui T me ka antibody IgM anti-human iole, a ua uhi mua ʻia ka hui C me ka anti-mouse antibody IgG antibody.
Strip A: Ke hāʻawi ʻia ka nui kūpono o ka mea hoʻāʻo i loko o ka pūnāwai hoʻāʻo o ka pahu hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.C.pneumoniae IgG antibody inā loaʻa i ka specimen e hoʻopaʻa i ka C. pneumoniae Antigen conjugates.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody IgG anti-human i uhi mua ʻia, e hana ana i kahi pūʻulu T kala burgundy,
e hōʻike ana i kahi hopena hoʻokolohua C. pneumoniae IgG maikaʻi.ʻO ka loaʻa ʻole o ka hui T e hōʻike ana i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti-mouse IgG/mouse IgGgold conjugate me ka nānā ʻole i ka loaʻa ʻana o ka hui T kala.A i ʻole, ka hopena hōʻike
ʻaʻole kūpono a pono e hoʻāʻo hou ʻia ka mea hoʻohālike me kahi mea hana ʻē aʻe.
Strip B: Ke hāʻawi ʻia ka nui o ka mea hoʻāʻo i loko o ka pūnāwai hoʻāʻo o ka cassette hoʻāʻo, neʻe ke kikoʻī ma o ka hana capillary ma o ka cassette.C.pneumoniae IgM antibody inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates C. pneumoniae Antigen.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody IgM anti-human IgM i uhi mua ʻia, e hana ana i kahi kāʻei T kala burgundy,
e hōʻike ana i kahi hopena hoʻokolohua C. pneumoniae IgM maikaʻi.ʻO ka loaʻa ʻole o ka hui T e hōʻike ana i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti-mouse IgG/mouse IgGgold conjugate me ka nānā ʻole i ka loaʻa ʻana o ka hui T kala.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.