KA HOIKE A ME KA HOAKAKA
ʻO ka maʻi maʻi Zika (Zika): lawe nui ʻia ma o ka nahu ʻana o ka makika Aedes, ka makuahine a me ke keiki, ka hoʻoili ʻana i ke koko a me ka hoʻouna ʻana i ka wahine.Hana ʻia ka antibody IgG/IgM i hoʻokahi pule ma hope o ka hoʻomaka ʻana, no laila he mea nui ka ʻike ʻana o IgG/IgM no ka wā mua.
ka hōʻailona o ka maʻi Zika.Ua ʻike ʻia ʻo Zika ma muli o ka nānā ʻana i ka serological a me ka hoʻokaʻawale viral i nā ʻiole a i ʻole ka moʻomeheu kiko.ʻO ka IgM immunoassay ke ʻano hana hoʻāʻo lab.Hoʻohana ka zika IgM/IgG Rapid Test i nā antigens recombinant i loaʻa mai kona ʻano protein, ʻike ʻo ia i ka anti-zika IgM/IgG i ka serum maʻi a i ʻole plasma i loko o 15 mau minuke.Hiki ke hana ʻia ka hoʻāʻo e nā limahana i hoʻomaʻamaʻa ʻole ʻia a liʻiliʻi paha, me ka ʻole o nā lako hana hoʻokolohua.
KUMU
ʻO ka Zika IgM/IgG Rapid Test he lateral flow chromatographic immunoassay.Aia i loko o ka pahu ho'āʻo: 1) kahi papa conjugate kala burgundy i loaʻa i nā antigen recombinant i hui pū ʻia me ke gula colloid (Zika conjugates) a me ka rabbit IgG-gold conjugates, 2) kahi membrane nitrocellulose i loaʻa i ʻelua mau pahu hoʻāʻo (M a me G band) a me kahi mana. kāʻei (C band).Hoʻopili mua ʻia ka M band me ka monoclonal anti-human IgM no ka ʻike ʻana i ka IgM anti-Zika, ua uhi mua ʻia ka band G me nā reagents no ka ʻike ʻana i ka IgG anti-Zika, a ua uhi ʻia ka hui C me ka anti-kao. rabbit IgG.
Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.ʻO Anti-Zika IgM inā loaʻa i ka specimen e paʻa i nā conjugates Zika.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody IgM anti-human pre-coated, e hana ana i kahi pahu M kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi Zika IgM.
ʻO Anti-Zika IgG inā loaʻa i ka specimen e paʻa i nā conjugates Zika.A laila hopu ʻia ka immunocomplex e nā reagents i uhi mua ʻia ma ka membrane, e hana ana i kahi band G color burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi Zika IgG.ʻO ka loaʻa ʻole o nā pūna hoʻāʻo (M a me G) hōʻike i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti rabbit IgG/rabbit IgG-gold conjugate me ka nānā ʻole i ka ulu ʻana o ka waihoʻoluʻu ma kekahi o nā kaula hoʻāʻo.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
