KA HOIKE A ME KA HOAKAKA
ʻO ka maʻi maʻi kekahi o nā kumu kumu o ka maʻi maʻi a me ka make ʻana o nā kamaliʻi ma ka honua holoʻokoʻa, ka hopena o 2.5 miliona mau make i kēlā me kēia makahiki.ʻO ka maʻi Rotavirus ke kumu nui o ka maʻi maʻi maʻi i nā pēpē a me nā keiki ma lalo o ʻelima mau makahiki, ʻo ia ka helu o 40% -60% o ka maʻi maʻi maʻi a me ka make ʻana o 500,000 mau keiki i kēlā me kēia makahiki.Ma ka makahiki ʻelima, kokoke i kēlā me kēia keiki o ka honua i loaʻa i ka rotavirus ma ka liʻiliʻi hoʻokahi.Me nā maʻi ma hope mai, loaʻa kahi pane ākea, heterotypic antibody;no laila, kakaʻikahi ka poʻe mākua.
I kēia lā ua hoʻokaʻawale ʻia nā hui ʻehiku o nā rotavirus (nā pūʻulu AG).
hōʻike ʻia.ʻO ka rotavirus hui A, ka rotavirus maʻamau, ke kumu ma mua o 90% o nā maʻi Rotavirus āpau i nā kānaka.Hoʻouna mua ʻia ʻo Rotavirus ma ke ala fecaloral, pololei mai ke kanaka a i ke kanaka.ʻO ka nui o ka nui o ka maʻi ma hope o ka hoʻomaka ʻana o ka maʻi, a laila emi iho.ʻO ka manawa incubation o ka maʻi rotavirus ka mea maʻamau i hoʻokahi a ʻekolu mau lā a hahai ʻia e ka gastroenteritis me ka awelika o ka lōʻihi o ʻekolu a ʻehiku lā.ʻO nā hōʻailona o ka maʻi mai ka maʻi maʻi a me ka wai a hiki i ka maʻi maʻi nui me ke kuni a me ka luaʻi.
Hiki ke ʻike ʻia kahi maʻi me ka rotavirus ma hope o ka hoʻomaʻamaʻa ʻana o ka gastroenteritis ke kumu o ka maʻi maʻi nui o nā keiki.I kēia mau lā, ua loaʻa ka ʻike kikoʻī o kahi maʻi me ka rotavirus ma o ka ʻike ʻana i ka antigen virus i loko o ka waihona e nā ʻano immunoassay e like me ka latex agglutination assay, EIA, a me ka lateral flow chromatographic immunoassay.
ʻO ka Rotavirus Ag Rapid Test he lateral flow chromatographic immunoassay e hoʻohana ana i ʻelua o nā antibodies kikoʻī e ʻike pono i ka antigen rotavirus i loko o ka fecal specimen.Hiki ke hana ʻia ka hoʻāʻo ʻana me ka ʻole o nā mea hana hoʻokolohua cumbersome, a loaʻa nā hopena i loko o 15 mau minuke.
KUMU
ʻO ka Rotavirus Ag Rapid Test he lateral flow chromatographic immunoassay.Aia ka pahu ho'āʻo: 1) kahi papa conjugate kala burgundy i loko o ka antibody anti-rotavirus monoclonal i hui pū ʻia me ke gula colloidal (conjugates anti-rotavirus) a me kahi antibody control i hui pū ʻia me ke gula colloidal, 2) kahi ʻāpana membrane nitrocellulose i loaʻa kahi laina hoʻāʻo (T laina) a me kahi laina mana (laina C).Hoʻopili mua ʻia ka laina T me kahi antibody anti-rotavirus monoclonal, a ua uhi mua ʻia ka laina C me kahi antibody laina mana.
Ke hāʻawi ʻia ka nui kūpono o ka mea hoʻohālike i unuhi ʻia i loko o ka pūnāwai laʻana o ka cassette hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.ʻO Rotavirus Ag, inā aia i loko o ka specimen, e hoʻopili i nā conjugates anti-rotavirus.A laila hopu ʻia ka immunocomplex ma ka membrane e ka antibody rotavirus pre-coated e hana ana i kahi laina T kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi rotavirus. e hōʻike ana i kahi hopena maikaʻi ʻole o ka rotavirus.Aia i loko o ka ho'āʻo kahi mana kūloko (laina C), pono e hōʻike i kahi laina kala burgundy o ka immunocomplex o nā antibodies hoʻomalu, me ka nānā ʻole i ka ulu ʻana o ka kala ma ka laina T.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
