KA HOIKE A ME KA HOAKAKA
ʻO Visceral leishmaniasis, a i ʻole Kala-azar, he maʻi i hoʻolaha ʻia e kekahi mau ʻano subspecies o ka L. donovani.Ua manaʻo ʻia ka maʻi e ka World Health Organization (WHO) e hoʻopilikia ma kahi o 12 miliona mau kānaka ma 88 mau ʻāina.Hoʻouna ʻia ia i ke kanaka ma o ka nahu ʻana o nā manu lele Phlebotomus, ka mea i loaʻa i ka maʻi mai ka hānai ʻana i nā holoholona i maʻi ʻia.ʻOiai he maʻi ia i loaʻa ma nā ʻāina ʻilihune, ma ʻEulopa Hema, ua lilo ia i mea maʻi nui loa i nā maʻi AIDS.ʻO ka ʻike ʻana i ke kino L. donovani mai ke koko, iwi iwi, ate, lymph nodes a i ʻole ka spleen e hāʻawi i kahi ʻano maopopo o ka maʻi.ʻIke serological o anti-L.ʻIke ʻia ʻo donovani IgM he māka maikaʻi loa no ka leishmaniasis Visceral acute.ʻO nā hoʻokolohua i hoʻohana ʻia ma ke keʻena keʻena e komo pū me ELISA, fluorescent antibody a i ʻole nā hoʻokolohua agglutination pololei 4-5.I kēia mau lā, ua hoʻomaikaʻi nui ka hoʻohana ʻana i ka protein L. donovani kikoʻī i ka hoʻāʻo ʻana i ka naʻau a me ka kikoʻī.
ʻO ka Leishmania IgG/IgM Combo Rapid Test he ho'āʻo serological e pili ana i ka protein recombinant, e ʻike ana i nā antibodies IgG a me IgM i ka L. Donovani i ka manawa like.Hāʻawi ka hōʻike i kahi hopena hilinaʻi i loko o 15 mau minuke me ka ʻole o nā mea hana.
KUMU
ʻO ka Leishmania IgG/IgM Rapid Test he lateral flow chromatographic immunoassay.Aia i loko o ka pahu ho'āʻo: 1) kahi papa conjugate kala burgundy i loaʻa i ka recombinant L. donovani antigen i hui pū ʻia me ke gula colloid (Leishmania conjugates) a me ka rabbit IgG-gold conjugates, 2) kahi ʻāpana nitrocellulose membrane i loaʻa i ʻelua mau pahu hoʻāʻo (T1 a me T2 band) a me kahi hui mana (C band).Hoʻopili mua ʻia ka hui T1 me ka monoclonal anti-human IgM no ka ʻike ʻana i ka anti-L.donovani IgM, T2 band ua precoated me reagents no ka ike ana i ka anti-L.donovani IgG, a ua uhi mua ʻia ka hui C me ke kao anti rabbit IgG.
Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.ʻO ka L. donovani IgM inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates Leishmania.A laila hopu ʻia ka immunocomplex ma ka membrane e ka antibody IgM anti-human pre-coated, e hana ana i kahi pūʻulu T1 kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi o L. donovani IgM.ʻO ka L. donovani IgG inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates Leishmania.A laila hopu ʻia ka immunocomplex e nā reagents i uhi mua ʻia ma ka membrane, e hana ana i kahi hui T2 kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi ʻo L. donovani IgG.
ʻO ka loaʻa ʻole o nā pūna T (T1 a me T2) hōʻike i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti rabbit IgG/rabbit IgG-gold conjugate me ka nānā ʻole i ka ulu ʻana o ka waihoʻoluʻu ma kekahi o nā kaula T.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
