KA HOIKE A ME KA HOAKAKA
Hoʻopili ʻia ʻo Helicobacter pylori me nā ʻano maʻi ʻōpū e like me ka dyspepsia non-ulcer, duodenal a me ka ʻōpū ʻōpū a me ka gastritis mau loa.Hiki ke hoʻonui i ka maʻi H. pylori ma mua o 90% i nā maʻi me nā hōʻailona a me nā hōʻailona o nā maʻi ʻōpū.Hōʻike nā haʻawina hou i kahi hui o ka maʻi H. pylori me ka maʻi maʻi ʻōpū.
pylori hiki ke hoʻoili ʻia ma o ka ʻai ʻana i ka meaʻai a i ʻole ka wai i haumia me ka fecal fecal.ʻO nā lāʻau antibiotic i hui pū ʻia me nā pūhui bismuth ua hōʻike ʻia he maikaʻi i ka mālama ʻana i ka maʻi H. Pylori ikaika..H.ʻIke ʻia ka maʻi pylori e nā ʻano hoʻāʻo invasive e pili ana i ka endoscopy a me ka biopsy (ʻo ia hoʻi ka histology, moʻomeheu) a i ʻole nā hana hoʻāʻo non-invasive e like me ka urea breath test (UBT), serologic antibody test and stool antigen test.Pono ʻo UBT i nā lako lab pipiʻi a me ka ʻai ʻana i kahi reagent radioactive.ʻAʻole ʻokoʻa nā hoʻāʻo antibody serologic ma waena o nā maʻi e hana nei i kēia manawa a me nā hōʻike i hala a i ʻole nā maʻi i hoʻōla ʻia.ʻO ka hoʻāʻo antigen stool e ʻike i ka antigen i loko o nā feces, kahi e hōʻike ai i kahi maʻi H. pylori ikaika.Hiki ke hoʻohana ʻia no ka nānā ʻana i ka pono o ka mālama ʻana a me ka hoʻi hou ʻana o kahi maʻi.pylori a me kekahi monoclonal anti-H.pylori e ʻike pono i ka H. pylori antigen i loko o ka fecal specimen o kahi maʻi maʻi.ʻO ka ho'āʻo he mea hoʻohana pono, pololei, a loaʻa ka hopena i loko o 15 mau minuke.
KUMU
ʻO ka H. pylori Ag Rapid Test kahi ʻano ʻāwīwī lateral flow chromatographic immunoassay.Aia ka pahu ho'āʻo: 1) kahi pad conjugate kala burgundy i loaʻa ka monoclonal anti-H.pylori antibody i hui pū ʻia me ke gula colloidal (anti-Hp conjugates) a me 2) kahi ʻāpana membrane nitrocellulose i loaʻa kahi laina hoʻāʻo (laina T) a me kahi laina mana (laina C).Ua uhi mua ʻia ka laina T me kekahi anti-H monoclonal.pylori, a ua uhi mua ʻia ka laina C me ka anti-mouse antibody IgG antibody.
Ke hāʻawi ʻia ka nui kūpono o ka mea hoʻoheheʻe fecal i unuhi ʻia i loko o ka pūnāwai laʻana o ka cassette hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.ʻO nā antigens H. pylori, inā aia i loko o ka specimen, e hoʻopaʻa ʻia i nā conjugates anti-Hp. A laila hopu ʻia ka immunocomplex ma ka membrane e ka antibody pre-coated e hana ana i kahi laina T kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi o H. pylori.ʻO ka loaʻa ʻole o ka laina T e hōʻike ana ʻo ka neʻe ʻana o nā antigens H. pylori i loko o ka specimen ma lalo o ka pae ʻike ʻia, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi ʻole H. pylori. Aia ka hōʻike i kahi mana kūloko (laina C) e hōʻike i kahi laina kala burgundy o ka immunocomplex o kao anti-iole IgG/mouse IgG-gold conjugate me ka nānā 'ole i ka ho'omohala kala ma ka laina T.Inā ʻaʻole e ulu ka laina C, ʻaʻole kūpono ka hopena o ka hoʻāʻo a pono e hoʻāʻo hou ʻia ka mea hoʻohālike me kahi mea ʻē aʻe.
