KA HOIKE A ME KA HOAKAKA
ʻO nā maʻi maʻi Dengue, he ʻohana o nā maʻi maʻi ʻehā ʻokoʻa o nā maʻi (Den 1,2,3,4), he mau maʻi maʻi RNA i hoʻopaʻa ʻia, envelop, maikaʻi.Lawe ʻia nā maʻi maʻi e nā makika o ka ʻohana Stegemyia nahu lā, ʻo ia hoʻi ʻo Aedes aegypti, a me Aedes albopictus.I kēia lā, ʻoi aku ma mua o 2.5 biliona poʻe e noho ana ma nā wahi o ʻAsia, ʻApelika, Australia, a me ʻAmelika e pilikia ana i ka maʻi dengue.Ua manaʻo ʻia he 100 miliona mau hihia o ka maʻi dengue a me 250,000 mau hihia o ka dengue hemorrhagic fever e hoʻoweliweli i ke ola i kēlā me kēia makahiki ma ke ao holoʻokoʻa.
ʻO ka ʻike serological o IgM antibody ke ala maʻamau no ka ʻike ʻana i ka maʻi maʻi dengue.I kēia mau lā, ʻike ʻia nā antigens i hoʻokuʻu ʻia i ka wā o ka hoʻopiʻi hou ʻana i ka maʻi maʻi i hōʻike i ka hopena maikaʻi loa.Hiki iā ia ke hōʻoia mai ka lā mua ma hope o ka hoʻomaka ʻana o ke kuni a hiki i ka lā 9, i ka pau ʻana o ka lāʻau lapaʻau o ka maʻi, no laila e hiki ai i ka mālama mua ʻana i hoʻokomo koke ʻia 4-. Hoʻokumu ʻia ka Dengue NS1 Rapid Test e ʻike i ka holo ʻana o ka antigen dengue i loko o ka serum. , plasma a i ʻole ke koko holoʻokoʻa.Hiki ke hana ʻia ka hoʻāʻo ʻana e nā limahana i hoʻomaʻamaʻa ʻole ʻia a i ʻole ka liʻiliʻi o ka mākaukau, me ka ʻole o nā lako hana.
KUMU
ʻO ka Dengue NS1 Rapid Test he lateral flow chromatographic immunoassay.Aia ka cassette ho'āʻo: 1) kahi papa conjugate kala burgundy i loaʻa i ka ʻiole anti-dengue NS1 antigen i hui pū ʻia me ke gula colloid (Dengue Ab conjugates), 2) kahi ʻāpana nitrocellulose membrane i loaʻa i kahi pahu hōʻike (T band) a me kahi pūʻali mana (C band).Hoʻopili mua ʻia ke kāʻei T me ka antigen anti-dengue NS1 antigen, a me ka hui C
ua uhi mua ʻia me ka anti-mouse IgG antibody.Hoʻomaopopo nā antibodies i ka antigen dengue i nā antigens mai nā serotype ʻehā o ka maʻi dengue.
Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette, neʻe ka mea hoʻohālike ma o ka hana capillary ma waena o ka cassette hoʻāʻo.ʻO Dengue NS1 Ag inā loaʻa i ka specimen e hoʻopaʻa i nā conjugates Dengue Ab.Hoʻopaʻa ʻia ka immunocomplex ma ka membrane e ka antibody antiNS1 i hoʻopaʻa mua ʻia, e hana ana i kahi kāʻei T kala burgundy, e hōʻike ana i kahi hopena hoʻokolohua Dengue Ag.
ʻO ka loaʻa ʻole o ka hui T e hōʻike ana i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti-mouse IgG/mouse IgG-gold conjugate me ka nānā ʻole i ke alo o ka hui T kala.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
