KA HOIKE A ME KA HOAKAKA
ʻO ka Cholera kahi maʻi maʻi maʻi maʻi i hōʻike ʻia e ka nui o ka nalowale ʻana o nā wai kino a me nā electrolytes ma muli o ka maʻi maʻi nui.Ua ʻike ʻia ka lāʻau etiological o ka maʻi kolera ʻo Vibrio cholerea (V. Cholerae), he hua bacteria gram negative, i hoʻouna ʻia i ke kanaka ma o ka wai a me ka meaʻai.
Hoʻokaʻawale ʻia ka ʻano V. Cholerae i kekahi mau serogroup ma ke kumu o nā antigens O.He hoihoi ko'iko'i nā pū'ulu 'O1 a me O139 no ka mea hiki iā lāua ke ho'oulu i ka ma'i ahulau a me ka ma'i kolera.He mea koʻikoʻi ka hoʻoholo koke ʻana i ka loaʻa ʻana o V. cholerae O1 a me O139 i loko o nā hōʻailona hōʻailona, wai, a me nā meaʻai i hiki ke mālama ʻia ka nānā pono a me nā hana pale kūpono e nā luna olakino.
Hiki ke hoʻohana pololei ʻia ka Cholera Ag Rapid Test ma ke kula e nā limahana i hoʻomaʻamaʻa ʻole ʻia a liʻiliʻi paha a loaʻa ka hopena ma lalo o 10 min, me ka ʻole o nā lako hana hoʻokolohua.
KUMU
ʻO ka Cholera Ag Rapid Test he lateral flow chromatographic immunoassay.Aia ka cassette ho'āʻo: 1) kahi pad conjugate kala burgundy i loaʻa ka monoclonal anti-V.Kolera O1 a me O139 antibodies i hui pū ʻia me ke gula colloid (O1/O139-antibody conjugates) a me ka rabbit IgG-gold conjugates, 2) he ʻāpana membrane nitrocellulose i loaʻa i ʻelua pahu hoʻāʻo (1 a me 139 mau pūʻali) a me kahi pūna mana (C band).Hoʻopili mua ʻia ka hui 1 me ka monoclonal anti-V.Kolera O1 antibody.Hoʻopili ʻia ka hui 139 me ka monoclonal anti-V.Kolera O139 antibody.Ua uhi mua ʻia ka hui C me ka anti-mouse IgG antibody.
Ke hoʻokomo ʻia ka nui kūpono o ka mea hoʻāʻo i loko o ka pūnāwai hoʻāʻo o ka cassette hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.V. Kolera O1/O139 antigen ina aia i loko o ka specimen e nakinaki i ka O1/O139-antibody gula conjugate.A laila hopu ʻia kēia immunocomplex ma ka membrane e ka anti-V i uhi mua ʻia.ʻO Cholera O1/O139 antibody, e hana ana i kahi hui hoʻāʻo kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi ʻo Cholera O1/O139.ʻO ka loaʻa ʻole o ka hui hōʻike hōʻike i kahi hopena maikaʻi ʻole.
Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti-mouse IgG/ʻiole IgG-gold conjugate me ka nānā ʻole i ka ulu ʻana o ka waihoʻoluʻu ma ka hui hoʻāʻo.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
