KA HOIKE A ME KA HOAKAKA
ʻO Chikungunya kahi maʻi maʻi maʻi kakaʻikahi i lawe ʻia e ka nahu o ka namu Aedes aegypti i maʻi ʻia.Hōʻike ʻia ia e ka ʻeha, ke kuni, a me ka ʻeha hui nui (arthralgias) e mau ana no ʻekolu a ʻehiku lā.Loaʻa ka inoa mai ka huaʻōlelo Makonde ʻo ia hoʻi "ka mea e kulou i luna" e pili ana i ke kūlana kūlou i ulu ʻia ma muli o nā hōʻailona o ka maʻi o ka maʻi.Hana ʻia i ka wā ua
ma nā wahi wela o ka honua, ma ʻApelika, Hema Hikina Hema, ʻĀina Hema a me Pakistan.ʻAʻole hiki ke ʻike ʻia nā hōʻailona ma ke ʻano o ka maʻi dengue.ʻOiaʻiʻo, ua hōʻike ʻia ka maʻi pālua o ka dengue a me ka chikungunya ma India.ʻAʻole e like me ka dengue, he kakaikahi nā hōʻike hemorrhagic a ʻo ka pinepine o ka maʻi he maʻi febrile palena ʻole.No laila he mea nui loa
e hoʻokaʻawale i ka dengue mai ka maʻi CHIK.ʻIke ʻia ʻo CHIK ma muli o ka loiloi serological a me ka hoʻokaʻawale viral i nā ʻiole a i ʻole ka moʻomeheu kiko.ʻO ka IgM immunoassay ke ʻano hana hoʻāʻo lab.Hoʻohana ka Chikungunya IgG/IgM Rapid Test i nā antigens recombinant i loaʻa mai kona ʻano protein, ʻike ia i ka IgG/IgM anti-CHIK i ka serum maʻi a i ʻole ka plasma i loko o 20 mau minuke.Hiki ke hana ʻia ka hoʻāʻo ʻana e ka mea i hoʻomaʻamaʻa ʻole ʻia
nā limahana akamai liʻiliʻi, me ka ʻole o nā lako hana hoʻokolohua.
KUMU
ʻO ka Chikungunya IgG/IgM+NS1 antigen Rapid Test he lateral flow chromatographic immunoassay.Aia ka pahu ho'āʻo me IgG/IgM Strip a me NS1 strip.
IgG/IgM strip:1) he pad conjugate kala burgundy i loaʻa nā antigens recombinant chikungunya i hui pū ʻia me ke gula colloid (conjugates dengue) a me nā conjugates IgG-gold rabbit, 2) kahi ʻāpana membrane nitrocellulose i loaʻa nā pahu hoʻāʻo ʻelua (ʻo G a me M band) a me kahi. pūʻulu mana (C band).Hoʻopili mua ʻia ka pūʻulu G me ka antibody no ka ʻike ʻana i ka maʻi virus IgG anti-Chikungunya, ua uhi ʻia ka hui M me ka antibody no ka ʻike ʻana i ka maʻi maʻi IgM anti-Chikungunya, a ua uhi ʻia ka hui C me ke kao anti rabbit IgG.
NS1 strip:1) he pad conjugate kala burgundy i loko o ka iole anti-Chikungunya NS1 antigen i hui pū ʻia me ke gula colloid (Chikungunya Ab conjugates), 2) he membrane nitrocellulose i loaʻa i ka pūna hoʻāʻo (T band) a me ka pūʻali mana (C band).Hoʻopili mua ʻia ke kāʻei T me ka anti-Chikungunya NS1 antigen, a ua uhi mua ʻia ka hui C me ka anti-mouse antibody IgG antibody.
IgG/IgM Strip: Ke hāʻawi ʻia ka nui o ka mea hoʻāʻo i loko o ka luawai hoʻāʻo o ka cassette hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma waena o ka cassette.ʻO ka maʻi IgG anti-Chikungunya inā loaʻa i ka specimen e paʻa i nā conjugates Chikungunya.A laila hopu ʻia ka immunocomplex e ka reagent i uhi ʻia ma ka band G, e hana ana i kahi kāʻei G kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi ʻo Chikungunya virus IgG a hōʻike i kahi maʻi hou a i ʻole hou.IgM anti-Chikungunya virus, inā aia i loko o ka specimen, e hoʻopaʻa i nā conjugates Chikungunya.A laila hopu ʻia ka immunocomplex e ka reagent i uhi mua ʻia ma ka band M, e hana ana i kahi hui M kala burgundy, e hōʻike ana i kahi hopena hoʻāʻo maikaʻi ʻo Chikungunya virus IgM a e hōʻike ana i kahi maʻi hou.ʻO ka loaʻa ʻole o nā pūʻulu hoʻāʻo (G a me M) e hōʻike ana i kahi hopena maikaʻi ʻole. Aia i ka hōʻike kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti rabbit IgG/
rabbit IgG-gold conjugate me ka nānā ʻole i ka ulu ʻana o ka waihoʻoluʻu ma kekahi o nā kaula T.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
NS1 Strip: Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette, neʻe ka mea hōʻike ma ka hana capillary ma waena o ka cassette hoʻāʻo.ʻO Chikungunya NS1 Ag inā loaʻa i ka specimen e paʻa i nā conjugates Chikungunya Ab.A laila, hopu ʻia ka immunocomplex ma ka membrane e ka antibody anti-NS1 rabbit precoated, e hana ana i kahi kāʻei T kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi ʻo Chikungunya Ag.ʻO ka loaʻa ʻole o ka hui T e hōʻike ana i kahi hopena maikaʻi ʻole.Aia i loko o ka ho'āʻo kahi mana kūloko (C band) pono e hōʻike i kahi pūʻulu kala burgundy o ka immunocomplex o kao anti-mouse IgG/mouse IgG-gold conjugate me ka nānā ʻole i ke alo o ka hui T kala.A i ʻole, ʻaʻole kūpono ka hopena hōʻike a pono e hoʻāʻo hou ʻia ka specimen me kahi mea ʻē aʻe.
